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Chromogenic Endotoxin Quantification Kit for Accurate Bacterial Toxin Measurement

Endotoxins, also known as lipopolysaccharides (LPS), are toxic components found in the outer membrane of Gram-negative bacteria. Accurate measurement of endotoxin levels is crucial in pharmaceutical, medical device, and biotechnology industries to ensure product safety and compliance with regulatory standards. The Chromogenic Endotoxin Quant Kit provides a reliable and sensitive method for quantifying endotoxin contamination.

Why Choose the Chromogenic Endotoxin Quant Kit?

This kit utilizes a chromogenic assay based on the Limulus Amebocyte Lysate (LAL) principle, which reacts specifically with bacterial endotoxins. The reaction produces a yellow-colored product that can be measured spectrophotometrically, allowing for precise quantification of endotoxin levels. Key advantages include:

• High sensitivity with a detection range of 0.005-1.0 EU/mL
• Excellent reproducibility and accuracy
• Simple and rapid procedure with results in 15-30 minutes
• Compatibility with various sample types (solutions, sera, and biological fluids)

Applications of the Chromogenic Endotoxin Quant Kit

The kit is widely used in multiple fields for endotoxin detection:

• Pharmaceuticals: Testing injectable drugs, vaccines, and medical devices
• Biotechnology: Monitoring cell culture media and recombinant proteins
• Research: Studying inflammatory responses and sepsis mechanisms
• Quality Control: Ensuring compliance with USP, EP, and JP standards

How to Use the Kit

The procedure involves three simple steps:

1. Sample Preparation: Dilute samples if necessary to fall within the detection range.
2. Reaction: Mix samples with the LAL reagent and incubate.
3. Measurement: Read absorbance at 405 nm and calculate endotoxin concentration using the provided standard curve.

For optimal results, follow the manufacturer’s instructions carefully and include appropriate controls in each assay.

Conclusion

The Chromogenic Endotoxin Quant Kit offers a fast, accurate, and user-friendly solution for endotoxin detection. Its high sensitivity and broad applicability make it an essential tool for researchers and quality control professionals working to ensure the safety of biological products and medical devices.